(X4) is the critical resistance gene for tigecycline degradation that has been continually reported in recent years. In particular, pathogenic bacteria carrying (X4) are a severe threat to human health. However, information describing coharboring (X4) with virulence genes is limited. Here, we isolated an strain coharboring (X4) and the heat-stable toxin gene from a dead piglet. The strain named 812A1-131 belongs to ST10. The genome was sequenced using the Nanopore and Illumina platforms. The virulence genes and (X4) are located on the chromosome and in the IncHI1-type plasmid p812A1-tetX4-193K, respectively. The plasmid could be conjugatively transferred to recipient J53 with high frequency. In vivo experiments showed that strain 812A1-131 is pathogenic to and could colonize the intestines of mice. In summary, pathogenic could receive a plasmid harboring the (X4) gene, which can increase the difficulty of treatment. The prevalence and transmission mechanisms of pathogenic bacteria coharboring the (X4) gene need more attention.
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| http://dx.doi.org/10.3390/pathogens12070903 | DOI Listing |
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