The performance of a commercial immunofluorescence assay (IFA commercial), an immunofluorescence assay (IFA ) and an indirect enzyme-linked immunosorbent assay (ELISA) were evaluated in the detection of antibodies anti- in the serum of Q fever patients and persons without the disease. For the study, seropositive and seronegative samples for Q fever ( = 200) from a serum bank of the Instituto Adolfo Lutz in Brazil were used. Commercial IFA was considered in this study as the gold standard for diagnosing Q fever. The IFA demonstrated good agreement with the commercial test, showing high sensitivity (91%) and specificity (97%) compared to the gold standard, with a Kappa coefficient of 0.8954. The indirect ELISA test showed lower agreement with the gold standard, showing low sensitivity (67%), although the specificity of the technique was high (97%) and the Kappa coefficient was moderate (0.6631). IFA is an excellent alternative for diagnosing Q fever.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10386034 | PMC |
| http://dx.doi.org/10.3390/pathogens12070873 | DOI Listing |
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