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Enhancing 1,3-Propanediol Productivity in the Non-Model Chassis through Genetic Manipulation. | LitMetric

Enhancing 1,3-Propanediol Productivity in the Non-Model Chassis through Genetic Manipulation.

Microorganisms

Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Av. Bandeirantes, 3900, Ribeirão Preto 14040-030, SP, Brazil.

Published: July 2023

AI Article Synopsis

  • * The study focuses on converting glycerol to 1,3-propanediol (1,3-PDO), a compound beneficial for creating solvents and organic acids, while discussing the challenges of using certain bacteria due to their limited genetic tools and slow growth.
  • * The researchers successfully overexpressed genes in a specific bacterial strain (Br21) to boost 1,3-PDO production and developed a method to enhance genetic manipulation options for this strain, enhancing its potential for bi

Article Abstract

Biotechnological processes at biorefineries are considered one of the most attractive alternatives for valorizing biomasses by converting them into bioproducts, biofuels, and bioenergy. For example, biodiesel can be obtained from oils and grease but generates glycerol as a byproduct. Glycerol recycling has been studied in several bioprocesses, with one of them being its conversion to 1,3-propanediol (1,3-PDO) by . is particularly interesting because it can produce a range of industrially relevant chemicals, including solvents and organic acids, and it is non-pathogenic. However, while species have many potential advantages as chassis for synthetic biology applications, there are significant limitations when considering their use, such as their limited genetic tools, slow growth rate, and oxygen sensitivity. In this work, we carried out the overexpression of the genes involved in the synthesis of 1,3-PDO in Br21, which allowed us to increase the 1,3-PDO productivity in this strain. Thus, this study contributed to a better understanding of the metabolic pathways of glycerol conversion to 1,3-PDO by a isolate. Also, it made it possible to establish a transformation method of a modular vector in this strain, therefore expanding the limited genetic tools available for this bacterium, which is highly relevant in biotechnological applications.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10383064PMC
http://dx.doi.org/10.3390/microorganisms11071855DOI Listing

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