Background: Our study aimed to examine the osteopontin (OPN) serum levels and tissue expression of CD44 and OPN in endometriosis-affected women both undergoing and not undergoing progestin treatment, and also to determine their involvement in the pathogenesis of endometriosis.
Methods: Using an ELISA kit, we evaluated the OPN serum levels of healthy and endometriosis-affected women both undergoing and not undergoing progestin treatment. Immunohistochemical (IHC) analyses were used to assess the endometriotic tissue expressions of CD44 and OPN.
Results: There were statistically significant higher OPN serum levels in the healthy control group compared to the women with endometriosis. Furthermore, there were higher OPN serum levels in the endometriosis-affected women undergoing the progestin treatment, but the difference did not reach statistical significance. In comparison to OPN, CD44 expression was significantly higher in all the endometriotic tissue glands and stroma, regardless of the patient's treatment status. Compared to the group receiving therapy, the OPN levels were higher in the endometriosis group not receiving therapy. OPN's robust cytoplasmic expression seemed to be associated with the non-treatment group.
Conclusion: Endometriosis, CD44, and OPN appear to be closely related. This study suggests that endometriosis that has not been treated has an immunological profile distinct to endometriosis that has received treatment.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10377117 | PMC |
| http://dx.doi.org/10.3390/biomedicines11072036 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Tetramethylpyrazine promotes osteo-angiogenesis during bone fracture repair.
J Orthop Surg Res
January 2025
Department of Hand and Foot Microsurgery, Jiangxi Careyou Shuguang Orthopedic Hospital, Jiayou Healthy City, No. 858 Fusheng Road, Xihu District, Nanchang, Jiangxi, 330002, China.
Background: Nonunion following a long bone fracture has gained a lot of attention due to the dreadful impact on the life quality of tremendous patients. Recent data have demonstrated the important involvement of angiogenesis in improving fracture healing. Tetramethylpyrazine (TMP) is an active component of Chinese herbal medicine with various biological activities including pro-angiogenesis property.
View Article and Find Full Text PDFOsteopontin Levels in Maternal Serum, Cord Blood, and Breast Milk According to Gestational Diabetes Mellitus: A Case-Control Study.
Nutrients
December 2024
Department of Social Pediatrics, Institute of Child Health, Hacettepe University, Ankara 06100, Turkey.
Background/objectives: The prevalence of gestational diabetes mellitus (GDM) is increasing, and GDM poses health risks for both mothers and newborns. This study investigated the association between GDM and two biomarkers, osteopontin (OPN) and betatrophin (ANGPTL8).
Methods: This study involved face-to-face interviews with 165 participants-81 in the GDM group and 84 in the control group-to gather maternal-newborn data.
Dichotomitin promotes osteoblast differentiation and improves osteoporosis by inhibiting oxidative stress.
J Orthop Surg Res
January 2025
Research Institute of Orthopedics, The Affiliated Jiangnan Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
Objective: Osteoporosis is a systemic disease with high morbidity and significant adverse effects. Increasing evidence supports the close relationship between oxidative stress and osteoporosis, suggesting that treatment with antioxidants may be a viable approach. This study evaluated the antioxidant properties of dichotomitin (DH) and its potential protective effects against osteoporosis.
View Article and Find Full Text PDFLINC01089 governs the miR-1287-5p/HSPA4 axis to negatively regulate osteogenic differentiation of mesenchymal stem cells.
Bone Joint Res
December 2024
Department of Orthopedics, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, China.
Aims: The involvement of long non-coding RNA (lncRNA) in bone marrow mesenchymal stem cell (MSC) osteogenic differentiation during osteoporosis (OP) development has attracted much attention. In this study, we aimed to disclose how LINC01089 functions in human mesenchymal stem cell (hMSC) osteogenic differentiation, and to study the mechanism by which LINC01089 regulates MSC osteogenesis.
Methods: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) and western blotting were performed to analyze LINC01089, miR-1287-5p, and heat shock protein family A (HSP70) member 4 (HSPA4) expression.
Ultrasensitive and label-free electrochemical immunosensor using gold nanoparticles deposited on a carbon electrode for the quantification of osteopontin: A serum-based oncomarker.
Int J Biol Macromol
December 2024
Department of Biosciences, Integral University, Lucknow 226026, India.
Early detection of cancer biomarkers is crucial for effective diagnosis and treatment, prompting the development of an ultrasensitive label-free electrochemical immunosensor. In this study, we fabricated an ultrasensitive label-free electrochemical immunosensor using a glassy carbon electrode/gold nanoparticles (GCE/AuNPs) modification for quantification of osteopontin (OPN), an oncomarker. The surface features of the modified electrodes were confirmed using scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS) methods.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!