Expression of housekeeping genes varies depending on mevalonate pathway inhibition in cancer cells.

Heliyon

Department of Biomedical Sciences, School of Biological and Environmental Sciences, Kwansei Gakuin University, 1 Gakuen Uegahara, Sanda, Hyogo 669-1330, Japan.

Published: July 2023

Statins have anticancer effects and may be used as anticancer agents via drug repositioning. In reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, the internal reference gene must not be affected by any experimental conditions. As statins exert a wide range of effects on cells by inhibiting the mevalonate pathway, it is possible that statin treatment might alter the expression of housekeeping genes used as internal reference genes, thereby misleading the assessment of obtained gene expression data. Here, we evaluated the expression stability of internal reference genes in atorvastatin-treated cancer cell lines. We treated both statin-sensitive and statin-resistant cancer cell lines with atorvastatin at seven different concentrations and performed RT-qPCR on 15 housekeeping genes whose expression stability was then assessed using five different algorithms. In both statin-sensitive and statin-resistant cancer cell lines, atorvastatin affected the expression of certain internal reference genes in a dose-dependent and cancer cell line-dependent manner; therefore, caution should be exercised when comparing target gene expression between cells. Our findings emphasize the importance of the validation of internal reference genes in gene expression analyses in drug treatment-based cancer research.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10368838PMC
http://dx.doi.org/10.1016/j.heliyon.2023.e18017DOI Listing

Publication Analysis

Top Keywords

internal reference
20
reference genes
16
cancer cell
16
housekeeping genes
12
gene expression
12
cell lines
12
expression
8
expression housekeeping
8
mevalonate pathway
8
expression stability
8

Similar Publications

Background: Effective healthcare delivery in today's diverse society necessitates healthcare providers' adeptness in navigating cultural and religious nuances in patient care. However, the integration of cultural competence training into medical education remains inadequate, particularly concerning the care of Muslim patients. In response, we introduce a novel educational intervention aimed at enhancing intercultural proficiency among medical students, emphasizing care for Muslim patients.

View Article and Find Full Text PDF

Mycotic aneurysm due to Magnusiomyces capitatus complicating a second liver transplant in a colonized patient.

Int J Infect Dis

December 2024

Institut des Agents Infectieux, Laboratoire de Parasitologie et Mycologie Médicale, Hospices Civils de Lyon, Lyon, France.; UR 3738-CICLY-Equipe Inflammation et Immunité de L'épithélium Respiratoire, Faculté de Médecine Lyon-Sud Charles Mérieux, Université Claude Bernard Lyon 1, Lyon, France.. Electronic address:

This report presents an extremely rare case of fungal mycotic aneurysm due to Magnusiomyces capitatus in a 51-year-old immunocompromised woman. The diagnosis was based on multiple CT-scans and various samples positive for the pathogen identified by sequencing of the Internal Transcribed Spacer region. Long-term treatment using caspofungin for previous candidemia would have promoted the dissemination of this intrinsically echinocandin-resistant fungus, from colonization sites in the lung and rectal areas.

View Article and Find Full Text PDF

Positive impact of DPP-4 or SGLT2 inhibitors on mild cognitive impairment in type 2 diabetes patients on metformin therapy: A metabolomic mechanistic insight.

Biomed Pharmacother

December 2024

Research and Innovation Hub, Alamein International University, Alamein, Egypt; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt; Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Alamein International University, Alamein, Egypt. Electronic address:

Mild cognitive impairment is increasingly recognized as a complication of type 2 diabetes (T2D). Although currently no disease-modifying treatments for cognitive disorders exist, interest surged in potential neuroprotective effects of newer anti-diabetic drugs. This study investigates the impact of newer anti-diabetic drug classes, dipeptidyl peptidase-4 (DPP-4i) and sodium-glucose cotransporter-2 inhibitors (SGLT2i) - on cognitive decline in T2D patients on metformin therapy.

View Article and Find Full Text PDF

Introduction: Recent international consensus statements advocate for the integration of Point-of-Care Ultrasound (PoCUS) into the global undergraduate medical curriculum. Some medical schools outside Saudi Arabia have already incorporated PoCUS into their undergraduate curricula to enhance anatomy, physiology and pathology instruction. However, there are no data on the potential role of PoCUS in the preclinical training of medical students in Saudi Arabia.

View Article and Find Full Text PDF

Adverse birth outcome research case definitions associated with maternal HIV and antiretroviral drug use in pregnancy: a scoping review.

BMC Pregnancy Childbirth

December 2024

Department of Paediatrics & Child Health, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.

Background: Adverse birth outcomes (preterm birth, low birth weight, small for gestational age, and stillbirth) seem to persist in infants born to people with HIV, even in the context of maternal antiretroviral therapy. However, findings have been disparate, inconclusive, and difficult to compare directly across settings, partly owing to variable outcome definitions. We aimed to collate, compare, and map existing adverse birth outcome definitions to inform a harmonized approach to universally measure these outcomes in studies including pregnant people with HIV.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!