AI Article Synopsis
- The study examines how 1,6-hexanediol (1,6HD) affects the nuclear pore complex (NPC) in live baker's yeast by disrupting its permeability barrier.
- After a short exposure to 1,6HD, the yeast cells showed no significant changes in growth or pH but exhibited effects on the cytoskeleton and a heat shock protein (Hsp104).
- The results indicate that 1,6HD allows larger proteins to pass into the nucleus and causes the displacement of various karyopherins, suggesting a mechanism involving the release of these proteins from the NPCs.
Article Abstract
Selective transport through the nuclear pore complex (NPC) depends on the dynamic binding of FG-repeat containing nucleoporins, the FG-nups, with each other and with Karyopherins (Kaps). Here, we assessed the specificity and mechanism by which the aliphatic alcohol 1,6-hexanediol (1,6HD) disrupts the permeability barrier of NPCs in live baker's yeast cells. After a 10-minute exposure to 5% 1,6HD, no notable changes were observed in cell growth, cytosolic pH and ATP levels, or the appearance of organelles. However, effects on the cytoskeleton and Hsp104 were noted. 1,6HD clearly affected the NPC permeability barrier, allowing passive nuclear entry of a 177kDa reporter protein that is normally confined to the cytosol. Moreover, multiple Kaps were displaced from NPCs, and the displacement of Kap122-GFP correlated with the observed passive permeability changes. 1,6HD thus temporarily permeates NPCs, and in line with Kap-centric models, the mechanism includes the release of numerous Kaps from the NPCs.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10376917 | PMC |
| http://dx.doi.org/10.1080/19491034.2023.2240139 | DOI Listing |
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