AI Article Synopsis
- T-cell location and activity are important predictors of how tumors respond to treatment, specifically in the context of pancreatic cancer (PDAC).
- Researchers used a special mouse model (TbiLuc) to track T-cells' behavior during PDAC tumor growth and treatment with specific immunotherapy drugs.
- Results showed significant differences in T-cell presence between tumors with high (hot) and low (cold) T-cell infiltration, and after treatment, an increase in T-cell activity was observed in lymph nodes, highlighting the model's effectiveness for monitoring immune responses.
Article Abstract
Introduction: The location of T-cells during tumor progression and treatment provides crucial information in predicting the response .
Methods: Here, we investigated, using our bioluminescent, dual color, T-cell reporter mouse, termed TbiLuc, T-cell location and function during murine PDAC tumor growth and checkpoint blockade treatment with anti-PD-1 and anti-CTLA-4. Using this model, we could visualize T-cell location and function in the tumor and the surrounding tumor microenvironment longitudinally. We used murine PDAC clones that formed tumors with either high T-cell infiltration (immunologically 'hot') or low T-cell infiltration (immunologically 'cold').
Results: Differences in total T-cell bioluminescence could be seen between the 'hot' and 'cold' tumors in the TbiLuc mice. During checkpoint blockade treatment we could see in the tumor-draining lymph nodes an increase in bioluminescence on day 7 after treatment.
Conclusions: In the current work, we showed that the TbiLuc mice can be used to monitor T-cell location and function during tumor growth and treatment.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10367003 | PMC |
| http://dx.doi.org/10.3389/fimmu.2023.1207533 | DOI Listing |
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