sp. nov., isolated from desert soil.

Int J Syst Evol Microbiol

State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.

Published: July 2023

Two Gram-stain-negative strains, designated as SYSU D00286 and SYSU D00782, were isolated from a sand sample collected from the Kumtag Desert in Xinjiang, north-west China. Cells were aerobic, non-motile and positive for both oxidase and catalase. Growth occurred at 4-37 °C (optimum, 28-30 °C), pH 6.0-7.0 (optimum, pH 7.0) and NaCl concentration of 0-1.5 % (w/v; optimum, 0%). Growth was observed on Reasoner's 2A agar and nutrient agar, but not on Luria-Bertani agar and trypticase soy agar. The polar lipids were identified as diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified aminolipids, one unidentified glycolipid and two unidentified phospholipids. The major respiratory quinone was ubiquinone-10 and the major fatty acids (>10 %) were C and summed feature 8 (C 7 and/or C 6). The 16S rRNA gene sequence similarity between strains SYSU D00286 and SYSU D00782 was 100%, and their average nucleotide identity (ANI), average amino acid identity and (AAI) digital DNA-DNA hybridization (dDDH) values were all 100.0 %. Phylogenetic analysis indicated that these two strains belong to the same species of the genus and show the highest sequence similarity to KCTC 72461 (98.2 %) and CCTCC AA 208029 (97.5 %). The ANI, AAI and dDDH values between SYSU D00286 (as well as SYSU D00782) and the other five type strains were all less than or equal to 83.2, 80.1 and 23.6 %, respectively. Based on their phylogenetic, phenotypic and chemotaxonomical features, strains SYSU D00286 and SYSU D00782 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SYSU D00286 (=MCCC 1K04981=CGMCC 1.8626=KCTC 82271).

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http://dx.doi.org/10.1099/ijsem.0.005990DOI Listing

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