Oil red O staining is effective in detection and quantification of neutral lipid droplets in tissues such as the liver. However, converting images into testable data using ImageJ can be time-consuming and is prone to inaccuracy or bias. We describe a protocol for automated qualitative measurement of lipid droplets in the bird liver using a batch processing macro script. We explain steps for extracting tissue, cryosectioning, staining, and imaging, followed by script generation for quantification of lipid in the images.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10382670 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102466 | DOI Listing |
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