AI Article Synopsis
- High-resolution respirometry enables the measurement of oxygen consumption in cells, tissues, and isolated mitochondria.
- The integrity of mitochondria is typically affected by cryopreservation, restricting assessments to fresh samples.
- This study introduces a straightforward method to evaluate mitochondrial respiratory complexes I and II in cryopreserved murine skeletal muscle and frozen D. melanogaster based on oxygen consumption.
Article Abstract
High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cryopreservation, these methods have been limited to fresh samples. Here we present a simple method to assess the activity of mitochondria respiratory complexes I and II in previously cryopreserved murine skeletal muscle tissue homogenates, as well as previously frozen D. melanogaster, as a function of oxygen consumption.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10365301 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0276147 | PLOS |
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