J Agric Food Chem
Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, China.
Published: August 2023
The ubiquitin-proteasome system (UPS) regulates protein quality or control and plays essential roles in several biological and biochemical processes in fungi. Here, we present the characterization of two UPS components, FonDoa1 and FonDoa4, in watermelon Fusarium wilt fungus, f. sp. (), and their biological functions. FonDoa1 localizes in both the nucleus and cytoplasm, while FonDoa4 is predominantly present in the cytoplasm. Both genes show higher expression in germinating macroconidia at 12 h. Deletion of or affects vegetative growth, conidiation, conidial germination/morphology, apoptosis, and responses to environmental stressors. , but not , positively regulates autophagy. The targeted disruption mutants exhibit significantly attenuated pathogenicity on watermelon due to defects in the infection process and invasive fungal growth. Further results indicate that the WD40, PFU, and PUL domains are essential for the function of FonDoa1 in pathogenicity and environmental stress responses. These findings demonstrate the previously uncharacterized biological functions of and in phytopathogenic fungi, providing potential targets for developing strategies to control watermelon Fusarium wilt.
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http://dx.doi.org/10.1021/acs.jafc.3c01785 | DOI Listing |
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