Herein, we provide a protocol for visualizing active osteoclast cathepsin K (CatK) with the quenched-fluorescent-activity-based probe qTJK17. We describe steps for isolating peripheral blood mononuclear cells, their differentiation into osteoclasts, and TRAP staining using an acid phosphatase leukocyte kit. We then detail visualization of active CatK. The probe qTJK17 includes a reactive group, acyloxymethylketone, that binds to the CatK active site, recognition sequence, and fluorescence donor-acceptor pair. This protocol can determine the exact localization of active CatK in osteoclasts. For complete details on the use and execution of this protocol, please refer to Janiszewski et al. (2023)..
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10374869 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102465 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Effects of Combined Antiemetic Protocol for the Prevention of Postoperative Nausea and Vomiting in Orthognathic Surgery: A Randomized Double Blinded Clinical Study.
J Oral Maxillofac Surg
December 2024
Professor, Private Practice, Proimtech A.Ş., Istanbul, Turkey.
Background: Postoperative nausea and vomiting (PONV) after orthognathic surgery remains one of the most common side effects despite the use of several medications.
Purpose: The study aimed to compare the frequencies of PONV between a combination of metoclopramide with granisetron and granisetron alone.
Study Design, Setting, Sample: A randomized double-blind clinical trial was conducted in 66 consecutive patients who underwent orthognathic surgery at the Department of Oral and Maxillofacial Surgery at Bezmialem Vakif University.
Consensus and Guidelines on Diagnosis and Management of Polypoidal Choroidal Vasculopathy (PCV) from the Asia-Pacific Vitreo-retina Society (APVRS).
Asia Pac J Ophthalmol (Phila)
January 2025
The Primasia International Eye Research Institute of The Chinese University of Hong Kong (Shenzhen), Shenzhen, China. Electronic address:
A panel of 21 international experts are formed by the Asia-Pacific Vitreo-retina Society to work out the consensus and guidelines on polypoidal choroidal vasculopathy (PCV). PCV is a common subtype of neovascular age-related macular degeneration and is more prevalent in Asian populations. Recent advancement in imaging technology allows greater understanding of the disease process of PCV.
View Article and Find Full Text PDFCompressed SENSE acceleration factor influence on magnetic resonance image quality in patients with endometrial cancer.
Phys Med
January 2025
Center for Radiology, University Clinical Center of Vojvodina, Hajduk Veljkova 1-9, 21000 Novi Sad, Serbia; Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. Electronic address:
Objectives: To investigate the impact of compressed sensing - sensitivity encoding (CS-SENSE) acceleration factor on the diagnostic performance of magnetic resonance imaging (MRI) within standard female pelvis protocol in patients with endometrial cancer.
Methods: T2-weighted turbo spin echo (TSE) sequence from standard female pelvic MRI protocol was chosen due to its long acquisition time and essential role in the evaluation of morphological characteristics of the female pelvic anatomical structures. Fully sampled reference scans and multiple prospectively 2x to 5x under-sampled CS-SENSE scans were acquired.
Burying poultry carcasses on farms as a disposal option in crisis situations: learnings and perspectives from a field study during an avian influenza epizootic in France.
Poult Sci
January 2025
Ploufragan-Plouzané-Niort Laboratory, Epidemiology Health and Welfare Unit, French Agency for Food, Environmental and Occupational Health and Safety (ANSES), BP53 22440 Ploufragan, France. Electronic address:
Appropriate disposal of dead farming animals is required to guarantee effective disease control while protecting the environment. In crisis situations, alternatives to rendering can be used, including on-farm burial. The objectives of this study were to: (i) describe the burial and monitoring protocols used on poultry farms in France in response to major avian influenza outbreaks; (ii) assess the effectiveness of the burial protocol, in terms of both technical and biosecurity aspects, and microbiological, physical and chemical changes of the buried materials and the environment over time; (iii) provide recommendations for future burial and follow-up protocols.
View Article and Find Full Text PDFProtocol to perform ex vivo electroretinography on adult zebrafish.
STAR Protoc
January 2025
Department of Ophthalmology & Visual Sciences, Medical College of Wisconsin Eye Institute, Milwaukee, WI 53226, USA. Electronic address:
Ex vivo electroretinography (ERG) provides insight into the health and functionality of retinal cells, the integrity of phototransduction, and the visual cycle and allows for the direct application of pharmaceuticals to the retinal tissues. Here, we present a protocol for performing ex vivo ERG on adult zebrafish. We describe steps for zebrafish tissue dissection, mounting tissues, and assembling and connecting cassettes.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!