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Incomplete filling of spray-dried KEDTA evacuated blood tubes: impact on measuring routine hematological parameters on Sysmex XN-10. | LitMetric

AI Article Synopsis

  • * Blood samples were collected from 17 healthy volunteers and dispensed in varying volumes (0.2 to 3.0 mL) into 3.0 mL KEDTA tubes, with tests conducted using the Sysmex XN-10 machine.
  • * Results showed clinically significant variations in tests from tubes filled below 67% of their capacity, indicating that such samples should be rejected to avoid interference with clinical decision making.

Article Abstract

Objectives: Because there is little published evidence on the effects of incomplete filling of KEDTA evacuated blood tubes on routine hematological testing, this original study aimed to provide updated information on this preanalytical aspect.

Methods: The study population consisted of 17 ostensibly healthy volunteers. Blood was drawn by venipuncture with a 10 mL syringe and dispensed in varying amounts (0.2, 0.5, 1.0, 2.0, and 3.0 mL) into 3.0 mL blood tubes containing spray-dried 5.4 mg KEDTA. All tubes were gently mixed and used to perform routine hematology tests on the Sysmex XN-10. Clinically significant variations were defined when the limits of desirable specifications of bias derived from biologic variation were exceeded.

Results: The desirable bias was exceeded in 33 % filled tubes (1.0 mL) for hematocrit and MCV (increased values) and for MCHC (decreased values), while it was exceeded in 17 % filled tubes (0.5 mL) for hemoglobin, hematocrit and MCV (increased values), and for MCHC (decreased values). Finally, the variation of values was higher than the desirable bias for RBC, hemoglobin, hematocrit and MCV (increase), and for MCHC and MPV (decrease) in 7 % filled tubes (0.2 mL). No clinically significant variations were observed in tubes filled up to 67 % of their nominal volume (i.e., 2.0 mL).

Conclusions: Consideration should be given to reject spray-dried KEDTA blood tubes that contain a blood volume <67 % of the nominal fill volume, as biased laboratory data in these samples may interfere with clinical decision making and care management.

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Source
http://dx.doi.org/10.1515/dx-2023-0064DOI Listing

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