Objectives: Adipogenesis is the fat cell formation process regulated by peroxisome proliferator-activated receptors (). The insulin-responsive glucose transporter 4 () has a major role in glucose uptake and metabolism in insulin target tissues (i.e., adipose and muscle cells). The interplay between and is essential for proper glucose homeostasis. This study aimed to isolate, elucidate, and investigate the effect of an isolated compound from XT6 on adipogenesis, , and expression in 3T3-L1 adipocytes.

Methods: The isolated compound was determined by analyzing spectroscopic data (LC-MS, FT-IR, Spectrophotometry UV-Vis, and NMR). The adipogenesis activity of the isolated compound in 3T3-L1 cells was determined by the Oil Red O staining method. RT-PCR was used to analyze the gene expression of and

Results: Di-(2-ethylhexyl)-phthalate (DEHP) was the isolated compound from XT6. The results revealed adipogenesis stimulation and inhibition, as well as and expressions

Conclusions: DEHP showed a non-monotonic dose-response (NMDR) effect on adipogenesis and and expression. It is the first study that reveals DEHP's NMDR effects on lipid and glucose metabolism in adipocytes.

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Source
http://dx.doi.org/10.1515/jcim-2023-0137DOI Listing

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