AI Article Synopsis
- Escherichia coli O157:H7 is a significant threat to human health, and traditional ELISA tests lack the sensitivity needed for its detection.
- A new method was created that enhances the detection of E. coli by utilizing a more efficient scaffold for alkaline phosphatase, resulting in improved fluorescence ELISA testing.
- The new fluorescence ELISA showed a detection limit of 3.75 × 10 cfu/mL and performed 6.16 times better than traditional methods, making it suitable for identifying dangerous bacteria in milk samples.
Article Abstract
Escherichia coli O157:H7 poses a threat to humans. Traditional ELISA is not a sensitive method for the detection of E. coli O157:H7. Here, an efficient method was designed for improving the load capacity of alkaline phosphatase (ALP) with streptavidin scaffolded DNA tetrad (SS-DNAt). With more ALP, more ascorbic acid 2-phosphate was catalyzed to ascorbic acid that was used to synthesize fluorescence poly adenine-thymine-templated copper nanoclusters. Based on SS-DNAt, fluorescence ELISA was successfully proposed for improving the sensitivity for detection of E. coli O157:H7 in milk samples. The method showed a linear range of 10 to 10 cfu/mL. The limit of detection of fluorescence ELISA was 3.75 × 10 cfu/mL and 6.16-fold better than that of traditional ELISA. The recovery of the fluorescence ELISA was 86.7 to 93.6% with the coefficient of variation of 5.6 to 10.5% in milk. This method could be used to detect hazardous material in food.
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| http://dx.doi.org/10.3168/jds.2022-23015 | DOI Listing |
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