AI Article Synopsis
- SARS-CoV-2 is a virus that makes people very sick, and it's important to find ways to control it globally.
- Scientists are now using special cells called monolayered intestinal epithelial cells, which come from human stem cells, to study how this virus infects people.
- These special cells have important factors that help the virus infect, and researchers found that blocking one of these factors can stop the virus from spreading in the cells.
Article Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes severe acute respiratory symptoms in humans. Controlling the coronavirus disease pandemic is a worldwide priority. The number of SARS-CoV-2 studies has dramatically increased, and the requirement for analytical tools is higher than ever. Here, we propose monolayered-intestinal epithelial cells (IECs) derived from human induced pluripotent stem cells (iPSCs) instead of three-dimensional cultured intestinal organoids as a suitable tool to study SARS-CoV-2 infection. Differentiated IEC monolayers express high levels of angiotensin-converting enzyme 2 and transmembrane protease serine 2 (TMPRSS2), host factors essential for SARS-CoV-2 infection. SARS-CoV-2 efficiently grows in IEC monolayers. Using this propagation system, we confirm that TMPRSS2 inhibition blocked SARS-CoV-2 infection in IECs. Hence, our iPSC-derived IEC monolayers are suitable for SARS-CoV-2 research under physiologically relevant conditions.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10354114 | PMC |
| http://dx.doi.org/10.1038/s41598-023-38548-1 | DOI Listing |
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