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Hydrogen Production from Barley Straw and Miscanthus by the Hyperthermophilic Bacterium, . | LitMetric

AI Article Synopsis

Article Abstract

This work aimed to evaluate the feasibility of biohydrogen production from Barley Straw and Miscanthus. The primary obstacle in plant biomass decomposition is the recalcitrance of the biomass itself. Plant cell walls consist of cellulose, hemicellulose, and lignin, which make the plant robust to decomposition. However, the hyperthermophilic bacterium, , can efficiently utilize lignocellulosic feedstocks (Barley Straw and Miscanthus) for energy production, and can now be metabolically engineered or isolated to produce more hydrogen and other biochemicals. In the present study, two strains, JWCB001 (wild-type) and JWCB018 (), were tested for their ability to increase hydrogen production from Barley Straw and Miscanthus. The JWCB018 resulted in a redirection of carbon and electron (carried by NADH) flow from lactate production to acetate and hydrogen production. JWCB018 produced ~54% and 63% more acetate and hydrogen from Barley Straw, respectively than its wild-type counterpart, JWCB001. Also, 25% more hydrogen from Miscanthus was obtained by the JWCB018 strain with 33% more acetate relative to JWCB001. It was supported that the engineered , such as the JWCB018, can be a parental strain to get more hydrogen and other biochemicals from various biomass.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619549PMC
http://dx.doi.org/10.4014/jmb.2305.05022DOI Listing

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