AI Article Synopsis
- Echinacoside (ECH) is a natural compound studied for its effects on fat metabolism and interaction with dopamine receptors in fat cells and mice.
- ECH significantly promoted fat cell "browning" in lab tests while reducing fat formation in living subjects, evidenced by increased expression of genes responsible for energy expenditure and thermogenesis.
- In animal models, ECH treatment resulted in a notable 12.28% decrease in body weight gain, highlighting its potential for obesity and metabolic syndrome management.
Article Abstract
Echinacoside (ECH) is a naturally occurring phenylethanoid glycoside, isolated from , and this study aimed to analyze its effect on thermogenesis and its interaction with dopaminergic receptors 1 and 5 (DRD1 and DRD5) in 3T3-L1 white adipocytes and mice models. We employed RT-PCR, immunoblot, immunofluorescence, a staining method, and an assay kit to determine its impact. ECH showed a substantial increase in browning signals in vitro and a decrease in adipogenic signals in vivo. Additionally, analysis of the iWAT showed that the key genes involved in beiging, mitochondrial biogenesis, and ATP-dependent thermogenesis were upregulated while adipogenesis and lipogenesis genes were downregulated. OXPHOS complexes, Ca signaling proteins as well as intracellular Ca levels were also upregulated in 3T3-L1 adipocytes following ECH treatment. This was collectively explained by mechanistic studies which showed that ECH mediated the beiging process the DRD1/5-cAMP-PKA and subsequent downstream molecules, whereas it co-mediated the α1-AR-signaling thermogenesis via the DRD1/5/SERCA2b/RyR2/CKmt pathway in 3T3-L1 adipocytes. Animal experiments revealed that there was a 12.28% reduction in body weight gain after the ECH treatment for six weeks. The effects of ECH treatment on adipose tissue can offer more insights into the treatment of obesity and metabolic syndrome.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10619551 | PMC |
| http://dx.doi.org/10.4014/jmb.2306.06041 | DOI Listing |
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