AI Article Synopsis
- * This study uses advanced imaging techniques in zebrafish to examine how RNA molecules are organized within the germ granules and finds that their positioning is influenced by translation activity and ribosome presence.
- * The vertebrate-specific protein Dead end (Dnd1) is crucial for the proper localization of nanos3 RNA, and when Dnd1 is absent or translation is blocked, this RNA shifts away from the active area, leading to a loss of germ cell identity, emphasizing the importance of compartmentalization in regulating germ cell fate.
Article Abstract
Germ granules, condensates of phase-separated RNA and protein, are organelles that are essential for germline development in different organisms. The patterning of the granules and their relevance for germ cell fate are not fully understood. Combining three-dimensional in vivo structural and functional analyses, we study the dynamic spatial organization of molecules within zebrafish germ granules. We find that the localization of RNA molecules to the periphery of the granules, where ribosomes are localized, depends on translational activity at this location. In addition, we find that the vertebrate-specific Dead end (Dnd1) protein is essential for nanos3 RNA localization at the condensates' periphery. Accordingly, in the absence of Dnd1, or when translation is inhibited, nanos3 RNA translocates into the granule interior, away from the ribosomes, a process that is correlated with the loss of germ cell fate. These findings highlight the relevance of sub-granule compartmentalization for post-transcriptional control and its importance for preserving germ cell totipotency.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10528888 | PMC |
| http://dx.doi.org/10.1016/j.devcel.2023.06.009 | DOI Listing |
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