sp. nov. and sp. nov., two novel bacteria isolated from the pit mud of Chinese liquor.

Two pink-pigmented bacterial strains, designated NBU2971 and NBU2972, were isolated from the pit mud of a Chinese liquor. Phylogenetic analyses based on 16S rRNA gene sequences suggested that strains NBU2971 and NBU2972 formed a distinct lineage within the family and were closely related to members of the genus . 16S rRNA gene sequences revealed that strain NBU2971 showed highest similarity of 97.9 % to 2b14, and strain NBU2972 showed the highest similarity of 96.9 % to JC215. The 16S rRNA gene sequence similarity, average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two novel strains were 95.2, 73.8 and 19.6 %, respectively, suggesting that they represent different species. The ANI and dDDH values between two novel strains and related species of genus were well below the threshold limit for prokaryotic species delineation. The genomic DNA G+C contents of strains NBU2971 and NBU2972 were 51.3 and 44.5 mol%, respectively. The major cellular fatty acids of the two novel strains were iso-C and summed feature 4 (iso-C I and/or anteiso-C B). The major polar lipid of both novel strains was phosphatidylethanolamine. The only respiratory quinone was MK-7. Combining results of phenotypic, chemotaxonomic and genotypic data, strains NBU2971 and NBU2972 are considered to be two representatives in the genus , which the name sp. nov. and sp. nov. are proposed. The type strains of the new species are NBU2971 (=KCTC 82916=MCCC 1K06395) and NBU2972 (=KCTC 82917=MCCC 1K06396), respectively.