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Dissecting components of the gene cluster in iron scavenging. | LitMetric

AI Article Synopsis

Article Abstract

is a leading cause of bacterial gastroenteritis worldwide. Acute infection can be antecedent to highly debilitating long-term sequelae. Expression of iron acquisition systems is vital for to survive the low iron availability within the human gut. The gene cluster is known to be upregulated during human infection and under iron limitation. While FetM and FetP have been functionally linked to iron transport in prior work, here we assess the contribution by each of the downstream genes ( ) to growth during both iron-depleted and iron-replete conditions. Significant growth impairment was observed upon disruption of , , and , suggesting a role in iron acquisition for each encoded protein. FetA expression was modulated by iron-availability but not dependent on the presence of FetB, FetC, FetD, FetE or FetF. Functions of the putative thioredoxins FetE and FetF were redundant in iron scavenging, requiring a double deletion (Δ ) to exhibit a growth defect. FetE was expressed and the structure solved to 1.50 Å, revealing structural similarity to thiol-disulfide oxidases. Functional characterization in biochemical assays showed that FetE reduced insulin at a slower rate than Trx and that together, FetEF promoted substrate oxidation in cell extracts, suggesting that FetE (and presumably FetF) are oxidoreductases that can mediate oxidation . This study advances our understanding of the contributions by the gene cluster to virulence at a genetic and functional level, providing foundational knowledge towards mitigating -related morbidity and mortality.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10350000PMC
http://dx.doi.org/10.1101/2023.07.05.547857DOI Listing

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