The bio-enzyme degradation technology is a promising approach to sustainably remove pollution in the water and laccase is one of the most widely used enzymes in this area. Nevertheless, the further industrial application of laccase is limited by low stability, short service, low reusability and high price. The immobilization technology can significantly improve the stability and reusability of enzymes and thus promoting their industrial applications. Nanocomposite materials have been developed and applied in the efficient immobilization of laccase due to their superior physical, chemical, and biological performance. This paper presents a comprehensive review of various nanocomposite immobilization methods for laccase and the consequent changes in enzymatic properties post-immobilization. Additionally, a comprehensive analysis is conducted on the factors that impact laccase immobilization and its water removal efficiency. Furthermore, this review examines the effectiveness of common contaminants' removal mechanisms while summarizing and discussing issues related to laccase immobilization on nanocomposite carriers. This review aims to provide valuable guidance for enhancing laccase immobilization efficiency and enzymatic water pollutant removal.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1007/s00449-023-02907-z | DOI Listing |
Int J Biol Macromol
January 2025
Department of Chemical Engineering, Toronto Metropolitan University 350 Victoria Street, Toronto, Ontario M5B 2K3, Canada. Electronic address:
The presence of endocrine disrupting chemicals (EDCs) in water can impart detrimental effects on public health by mimicking the behaviors of natural hormones and their associated receptors in human body. Studies have demonstrated that ligninolytic enzymes such as laccase can degrade various phenolic compounds, including a broad range of EDCs. In this study, the technique of covalent immobilization of laccase through carbodiimide coupling chemistry on highly adsorptive reduced graphene oxide (rGO) sponges was utilized to effectively remove two representative EDCs; namely, bisphenol A (BPA) and triclosan (TCS) from water.
View Article and Find Full Text PDFSensors (Basel)
December 2024
Division de Fotónica, Centro de Investigaciones en Óptica AC, Loma del Bosque 115, Col. Lomas del Campestre, León 37150, Guanajuato, Mexico.
Methylene blue is a cationic organic dye commonly found in wastewater, groundwater, and surface water due to industrial discharge into the environment. This emerging pollutant is notably persistent and can pose risks to both human health and the environment. In this study, we developed a Surface Plasmon Resonance Biosensor employing a BK7 prism coated with 3 nm chromium and 50 nm of gold in the Kretschmann configuration, specifically for the detection of methylene blue.
View Article and Find Full Text PDFAntioxidants (Basel)
December 2024
Dipartimento di Chimica "Giacomo Ciamician", Alma Mater Studiorum-Università di Bologna, Via Gobetti 83, 40129 Bologna, Italy.
This review highlights the progress made in recent years on biosensors aimed at detecting relevant analytes/markers of food peroxidation. Starting from the basic definition of biosensors and the chemical features of peroxidation, here we describe the different approaches that can be used to obtain information about the progress of peroxidation and the efficacy of antioxidants. Aptamers, metal-organic frameworks, nanomaterials, and supported enzymes, in conjunction with electrochemical methods, can provide fast and cost-effective detection of analytes related to peroxidation, like peroxides, aldehydes, and metals.
View Article and Find Full Text PDFInt J Biol Macromol
January 2025
Department of Pharmaceutical Technology, National Institute of Pharmaceutical Education and Research (NIPER) S.A.S. Nagar, Mohali, Punjab 160062, India. Electronic address:
Customized nano-biocatalysts of laccase have been made using nano-structured polyaniline viz. nano-fibers and nano-tubes, as immobilization supports and a simultaneous comparison between them has been made. Laccases are poly-phenol oxidases having tremendous utility concerning wider areas of application especially in the field of organic and drug syntheses.
View Article and Find Full Text PDFSci Rep
January 2025
Faculty of Biotechnology, German International University, Regional Ring Road, East Cairo, New Administrative Capital, Cairo, Egypt.
In the current study, calcium alginate was used as a carrier for Agaricus bisporus CU13 laccase immobilization, with an immobilization yield of the entrapped laccase of 91.95%. Free and immobilized enzymes showed their best enzyme activity at 60 °C as an optimum temperature.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!