AI Article Synopsis
- AC16 cells, a human cardiac cell line, are useful for studying heart cell biology, particularly senescence and proliferation.
- Researchers found that low serum conditions and treatments like low-dose doxorubicin, UV light, or hydrogen peroxide can induce reduced cell growth and senescence markers in these cells.
- Key indicators of senescence include increased levels of p21 and H2AX, but not p16, while proteomics analysis revealed a loss of histones and increased thymidine kinase expression associated with doxorubicin treatment.
Article Abstract
AC16 cells are a transformed human cardiac cell line commonly used to study cardiomyocyte biology. We show that reduced proliferation and senescence markers can be robustly induced in AC16 cells cultured in low serum condition and treated with (i) low-dose doxorubicin, (ii) UV 254 nm, or (iii) H O exposure for up to 48 hours. Increased p21 (CDKN1A) and H2A.X variant histone (H2AX) levels serve as reliable molecular markers upon all three treatment conditions, but the up-regulation of another common senescence marker, p16 (CDKN2A) was not observed. A proteomics screen further shows that the loss of histones and the increased expression of thymidine kinases (TK1) are prominent features of AC16 cells under doxorubicin induced senescence.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10346100 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.000865 | DOI Listing |
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