At present, most studies have shown that probiotics have a positive regulatory effect on the nutritional metabolism of the body, but the mechanism is still unclear. Here, 48 piglets were divided into four groups. The control group was not fed probiotics, the Lac group was fed GG ATCC53103, the Rha group was fed JL01, and the mix group was fed two types of probiotics. Nitrogen metabolism and mRNA levels of mTOR and S6K in skeletal muscle were observed in each group. Then, metagenome and non-targeted metabonomics were used to observe the changes of intestinal microorganisms and plasma metabolites in portal channels after probiotics feeding. Finally, we combined the results of omics analysis to reveal the mechanism of probiotics on nitrogen metabolism in weaned piglets. The results showed that GG ATCC53103 and JL01 increased nitrogen apparent digestibility, nitrogen deposition rate, and nitrogen utilization rate of weaned piglets ( < 0.05); the relative expression of mTOR and SK6 mRNA in skeletal muscle increased significantly ( < 0.05). When GG ATCC53103 and JL01 were combined, we found that and significantly increased in the jejunum ( < 0.05). The relative abundance of , and in the ileum increased significantly ( < 0.05). Compared with the control group, L-Tryptophan, 3-Phosphonyloxypyruvate, cis-Aconitate, and Carbamoyl phosphate were significantly increased in the mixed group portal vein. The result of the combinatorial analysis showed that the significantly increased microorganisms could encode the enzyme genes for the synthesis of L-Tryptophan, 3-Phosphonooxypyruvate, cis-Aconitate, and Carbamoyl phosphate. In summary, our results demonstrated that GG ATCC53103 and JL01 could stimulate the expression of skeletal muscle protein synthesis genes of weaned piglets by modulating the structure of the gut microbiota and its metabolites, thereby improving nitrogen metabolism in weaned piglets.
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http://dx.doi.org/10.3389/fmicb.2023.1200594 | DOI Listing |
Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of the feed additive consisting of endo-1,3(4)-beta-glucanase and endo-1,4-beta-xylanase (produced with IMI CC 378536) (Rovabio® Excel) for the renewal of its authorisation as a zootechnical feed additive for all poultry species, weaned piglets, pigs for fattening and sows. The applicant provided evidence that the additive complies with the conditions of the authorisation. The FEEDAP Panel concluded that the additive in all its formulations remains safe for all poultry species, weaned piglets, pigs for fattening and sows, and remains safe for consumers and the environment.
View Article and Find Full Text PDFGenomics
January 2025
Microbe Interactomics Group, Dept. Animal Sciences, Wageningen University & Research (WUR), Wageningen, the Netherlands. Electronic address:
This study investigates the impact of maternal gestation diets with varying fiber contents on gene expression and chromatin accessibility in fetuses and piglets fed a low fiber diet post weaning. High-fiber maternal diets, enriched with sugar beet pulp or pea internal fiber, were compared to a low-fiber maternal diet to evaluate their effects on liver and muscle tissues. The findings demonstrate that maternal high-fiber diets significantly alter chromatin accessibility, predicted transcription factor activity and transcriptional landscape in both fetuses and piglets.
View Article and Find Full Text PDFRes Vet Sci
December 2024
Metabolic Modifiers for Aquaculture, Agricultural Biotechnology Department, Center for Genetic Engineering and Biotechnology (CIGB), 31st Avenue and 190, Havana 10600, Cuba. Electronic address:
Animals (Basel)
January 2025
Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Phuttamonthon, Nakhon Pathom 73170, Thailand.
Porcine epidemic diarrhea (PED) virus is an important cause of diarrhea in sows and piglets [...
View Article and Find Full Text PDFAnimals (Basel)
December 2024
School of Agriculture and Food Science, University College Dublin, Belfield, D04 W6F6 Dublin, Ireland.
Weaning in piglets presents significant physiological and immunological challenges, including gut dysbiosis and increased susceptibility to post-weaning diarrhoea (PWD). Abrupt dietary, environmental, and social changes during this period disrupt the intestinal barrier and microbiota, often necessitating antimicrobial use. Sustainable dietary strategies are critical to addressing these issues while reducing reliance on antimicrobials.
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