In this investigation, two new thermophilic bacteria were isolated. The new isolates were characterized by 16S rRNA, biochemical, morphological, and physiological analyzes and the isolates were identified as strain Gecek20 and thermophilic strain Gecek19. Various biological activities of extracellular Ag-NPs synthesized from thermophilic strain Gecek20 and thermophilic strain Gecek19 were evaluated. The produced NPs were analyzed by SEM, SEM-EDX, and XRD analyses. The antioxidant abilities of new synthesized Ag-NPs from thermophilic strain Gecek20 (T1-Ag-NPs) and new synthesized Ag-NPs from thermophilic strain Gecek19 (T2-Ag-NPs) were studied by DPPH inhibition and metal chelating ability. The highest DPPH and metal chelating abilities of T1-Ag-NPs and T2-Ag-NPs at 200 mg/L concentration were 93.17 and 90.85%, and 75.80 and 83.64%, respectively. The extracellular green synthesized T1-Ag-NPs and T2-AgN-Ps showed DNA nuclease activity at all tested concentrations. Moreover, both new synthesized Ag-NPs had antimicrobial activity against the strains studied, especially on Gram positive bacteria. T1-Ag-NPs and T2-AgNPs also showed powerful growth inhibition. The highest biofilm inhibition percentages of T1-Ag-NPs and T2-Ag-NPs against and were 100.0%, respectively, at 500 mg/L.
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http://dx.doi.org/10.1080/10826068.2023.2230496 | DOI Listing |
BMC Genomics
December 2024
Department of Biological Sciences, University of Bergen, Bergen, N-5020, Norway.
Background: Fervidobacterium is a genus of thermophilic anaerobic Gram-negative rod-shaped bacteria belonging to the phylum Thermotogota. They can grow through fermentation on a wide range of sugars and protein-rich substrates. Some can also break down feather keratin, which has significant biotechnological potential.
View Article and Find Full Text PDFBioresour Technol
December 2024
Leibniz Institute for Agricultural Engineering and Bioeconomy e. V. (ATB), Department Microbiome Biotechnology, Max-Eyth-Allee 100, Potsdam 14469, Germany. Electronic address:
Lignocellulosic media, containing diverse sugars and growth inhibitor compounds, pose great challenges to fermentation processes. This study tested thermophile Heyndrickxia coagulans strains for the production of L-(+)-lactic acid from waste wood hydrolysate. H.
View Article and Find Full Text PDFFEMS Microbiol Ecol
December 2024
University of Iceland, Department of Computer Science, School of Engineering and Natural Sciences, Dunhagi 5, 107 Reykjavik, Iceland.
The thermophilic bacterium Rhodothermus marinus has mainly been studied for its thermostable enzymes. More recently, the potential of using the species as a cell factory and in biorefinery platforms has been explored, due to the elevated growth temperature, native production of compounds such as carotenoids and EPSs, the ability to grow on a wide range of carbon sources including polysaccharides, and available genetic tools. A comprehensive understanding of the metabolism of cell factories is important.
View Article and Find Full Text PDFMicrobiol Resour Announc
December 2024
Microbial Genetic Division, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
GBlys is a lysogenic strain of the temperate phage phiOH2, which is 3,644,428 bp long, has a GC content of 52%, and contains 3,595 predicted protein-coding genes. Here, we report a resequenced GBlys genome obtained by deep sequencing with long and short reads.
View Article and Find Full Text PDFEnviron Microbiol
December 2024
AgResearch Limited, Hopkirk Research Institute, Palmerston North, New Zealand.
This study investigated the diversity of thermophilic Campylobacter species isolated from three New Zealand freshwater catchments affected by pastoral and urban activities. Utilising matrix-assisted laser desorption ionisation-time of flight and whole genome sequence analysis, the study identified Campylobacter jejuni (n = 46, 46.0%), C.
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