AI Article Synopsis
- Mapping protein-protein interactions is important for understanding how proteins function in biological systems.
- Recent techniques like proximity-dependent biotinylation (BioID) combined with mass spectrometry (MS) have improved our ability to study protein complexes in various plant models.
- This study focuses on using BioID in tomato hairy root cultures and offers guidance on analyzing the mass spectrometry data generated from this process.
Article Abstract
Mapping protein-protein interactions is crucial to understand protein function. Recent advances in proximity-dependent biotinylation (BioID) coupled to mass spectrometry (MS) allow the characterization of protein complexes in diverse plant models. Here, we describe the use of BioID in hairy root cultures of tomato and provide detailed information on how to analyze the data obtained by MS.
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| http://dx.doi.org/10.1007/978-1-0716-3327-4_26 | DOI Listing |
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