Co-immunoprecipitation-Based Identification of Effector-Host Protein Interactions from Pathogen-Infected Plant Tissue.

Methods Mol Biol

Department of Plant Pathology, Institute of Crop Science and Resource Conservation (INRES), University of Bonn, Bonn, Germany.

Published: July 2023

Protein-protein interactions play an essential role in host-pathogen interactions. Phytopathogens secrete a cocktail of effector proteins to suppress plant immunity and reprogram host cell metabolism in their favor. Identification and characterization of effectors and their target protein complexes by co-immunoprecipitation can help to gain a deeper understanding of the functions of individual effectors during pathogenicity and can also provide new insights into the wiring of plant signaling pathways or metabolic complexes. Here we describe a detailed protocol to perform co-immunoprecipitation of effector-target protein complexes from plant extracts with an example of the Ustilago maydis/maize pathosystem for which we also provide a fungal protoplast transformation and maize seedling infection protocols.

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http://dx.doi.org/10.1007/978-1-0716-3327-4_8DOI Listing

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