AI Article Synopsis
- Sepsis is a serious condition caused by an improper response to infection, and timely diagnosis using procalcitonin (PCT) is vital in healthcare.
- The International Federation of Clinical Chemistry is working on standardizing PCT testing using a new reference method and high-quality materials to improve diagnostic accuracy.
- The study found that magnetic-core nano-particles with specific antibodies are the most effective for isolating PCT, enhancing both its quantification and detection in clinical samples.
Article Abstract
Sepsis is a life-threatening organ dysfunction caused by a dysregulated host response to infection. The fast and accurate diagnosis of sepsis by procalcitonin (PCT) has emerged as an essential tool in clinical medicine. Although in use in the clinical laboratory for a long time, PCT quantification has not yet been standardized. The International Federation of Clinical Chemistry working group on the standardization of PCT (IFCC-WG PCT) aims to provide an LC-MS/MS-based reference method as well as the highest metrological order reference material to address this diagnostic need. Here, we present the systematic evaluation of the efficiency of an immuno-enrichment method, based on functionalized Sepharose, magnetic-core, or polystyrene (latex) nano-particles, to quantitatively precipitate PCT from different human sample materials. This method may be utilized for both mass spectrometric and proteomic purposes. In summary, only magnetic-core nano-particles functionalized by polyclonal PCT antibodies can fulfil the necessary requirements of the international standardization of PCT. An optimized method proved significant benefits in quantitative and specific precipitation as well as in the subsequent LC-MS/MS detection of PCT in human serum samples or HeLa cell extract. Based on this finding, further attempts of the PCT standardization process will utilize a magnetic core-derived immuno-enrichment step, combined with subsequent quantitative LC-MS/MS detection.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10341910 | PMC |
| http://dx.doi.org/10.3390/ijms241310963 | DOI Listing |
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