NMR-Based Characterization of Wood Decay Fungi as Promising Novel Foods: , and Mycelia as Case Studies.

Wood Decay Fungi (WDF) are fungi specialized in degrading wood. An interesting perspective is their use as a source of Novel Foods or food ingredients. Here, for the first time, the metabolite profiling of hydroalcoholic and organic extracts from , , mycelia was investigated by NMR methodology. Amino acids (alanine, arginine, asparagine, aspartate, betaine, GABA, glutamate, glutamine, histidine, isoleucine, leucine, lysine, phenylalanine, threonine, tryptophan, tyrosine, valine), sugars (galactose, glucose, maltose, trehalose, mannitol), organic acids (acetate, citrate, formate, fumarate, lactate, malate, succinate), adenosine, choline, uracil and uridine were identified and quantified in the hydroalcoholic extracts, whereas the H spectra of organic extracts showed the presence of saturated, mono-unsaturated and di-unsaturated fatty chains, ergosterol,1,2-diacyl--glycero-3-phosphatidylethanolamine, and 1,2-diacyl-glycero-3-phosphatidylcholine. extracts showed the highest amino acid concentration. Some compounds were detected only in specific species: betaine and mannitol in , maltose in , galactose in , GABA in and , and acetate in and . showed the highest saturated fatty chain concentration, whereas DUFA reached the highest concentration in A high amount of ergosterol was measured both in and . The reported results can be useful in the development of WDF-based products with a high nutritional and nutraceutical value.