We report an Osprey-based computational protocol to prospectively identify oncogenic mutations that act via disruption of molecular interactions. It is applicable to analyse both protein-protein and protein-DNA interfaces and it is validated on a dataset of clinically relevant mutations. In addition, it is used to predict previously uncharacterised patient mutations in CDK6 and p16 genes, which are experimentally confirmed to impair complex formation.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344873PMC
http://dx.doi.org/10.1038/s42003-023-05089-2DOI Listing

Publication Analysis

Top Keywords

protein-protein protein-dna
8
disruptor computational
4
computational identification
4
identification oncogenic
4
oncogenic mutants
4
mutants disrupting
4
disrupting protein-protein
4
protein-dna interactions
4
interactions report
4
report osprey-based
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!