Glutathione Coating of Liposomes Enhances the Delivery of Hydrophilic Cargo to the Inner Nuclear Layer in Retinal Cultures.

To successfully deliver intracellular compounds to retinal cells, a delivery system based on purified lipids, self-assembled into synthetic vesicles called liposomes, can be used. Liposomes have the potential to target distinct tissues and cells in the body by molecular targeting moieties conjugated to their surface. To enhance liposome delivery to neurons, glutathione has formerly been used as targeting moiety. It is unclear whether and how the glutathione conjugation improves the liposome-induced uptake to cells within the retina. To explore this, glutathione-liposomes were prepared and loaded with a fluorescent tracer, which was added to organotypic retinal explant cultures derived from mice. The fluorescence in the tissue was analyzed from histological sections using fluorescent microscopy. Comparisons were done with liposomes without a targeting device and cysteine-conjugated liposomes. A significant increase (p ≤ 0.05) of fluorescent signal was observed from the inner nuclear layer of retinas exposed to glutathione-conjugated liposomes. Qualitatively, this might be attributed to the accumulation of glutathione-liposomes in the retinal inner vasculature, but further studies are needed for verification.