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Organic Extraction of Nucleic Acids Using Ethanol Precipitation or Microcon Centrifugal Filter Purification Methods. | LitMetric

AI Article Synopsis

  • - Organic solvents are widely used for separating nucleic acids in molecular biology and biochemistry, especially for isolating DNA from difficult samples like skeletal remains.
  • - Forensic DNA analysis benefits from organic extraction methods, as they help purify DNA from samples that may be degraded or contain contaminants that hinder further analysis.
  • - The chapter outlines techniques for effectively extracting and purifying DNA from challenging forensic samples to improve the chances of obtaining clear short tandem repeat (STR) profiles.

Article Abstract

The use of organic solvents to separate nucleic acids from other cell components is a common practice among many scientific fields, including molecular biology and biochemistry. The advantage of performing organic extractions in forensic DNA analysis is the ability to purify DNA from heavily degraded or inhibitory sample types, such as skeletal remains. These sample types require special care to ensure that the DNA is contaminant-free since they often contain PCR inhibitors that negatively impact downstream DNA analysis, resulting in unobtainable or uninterpretable short tandem repeat (STR) profiles. Purification of DNA after an organic isolation procedure is essential for improving the likelihood of obtaining valid STR profile from a challenging evidence sample. This chapter describes the methodology for extracting and purifying DNA from various types of challenging samples that are often encountered in forensic casework.

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Source
http://dx.doi.org/10.1007/978-1-0716-3295-6_2DOI Listing

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