The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70A, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70A was a Gram-positive, strictly anaerobic, motile, rod-shaped bacterium. Formate, but not acetate, was a fermentative end-product from growth in peptone-yeast extract and peptone-yeast-glucose broth. The G+C content of DNA from strain CC70A was 34.9 mol%. 16S rRNA gene sequence analysis revealed that the isolate was part of the phylum . The closest described relatives of strain CC70A were (93.3 %) and (93.3 and 91.9% sequence similarity across 16S rRNA gene, respectively). According to the data obtained in this work, strain CC70A represents a novel bacterium belonging to a new genus for which the name gen. nov., sp. nov. is proposed. The type strain for our described novel species is CC70A (=DSM 27931= JCM 30568).
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http://dx.doi.org/10.1099/ijsem.0.005958 | DOI Listing |
Int J Syst Evol Microbiol
July 2023
Department of Microbiology and Plant Biology, University of Oklahoma, Oklahoma, USA.
The diversity of bacteria associated with biopsy material obtained from patients with colorectal cancer was investigated using culture techniques. A novel bacterium, strain CC70A, was isolated by diluting a sample of homogenized tissue in anaerobic medium, and then plating to yield a pure culture. Strain CC70A was a Gram-positive, strictly anaerobic, motile, rod-shaped bacterium.
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