AI Article Synopsis

  • Immunodominance of non-neutralizing antibodies and somatic hypermutation in germinal centers pose challenges for creating an effective HIV vaccine.
  • Researchers used implantable osmotic pumps to deliver targeted immunogens to rhesus macaques over six months, aiming to stimulate immune responses against a conserved fusion peptide.
  • Methods like electron microscopy polyclonal epitope mapping (EMPEM) and lymph node analysis tracked antibody responses, helping identify key residues for future vaccine design.

Article Abstract

Immunodominance of antibodies targeting non-neutralizing epitopes and the high level of somatic hypermutation within germinal centers (GCs) required for most HIV broadly neutralizing antibodies (bnAbs) are major impediments to the development of an effective HIV vaccine. Rational protein vaccine design and non-conventional immunization strategies are potential avenues to overcome these hurdles. Here, we report using implantable osmotic pumps to continuously deliver a series of epitope-targeted immunogens to rhesus macaques over the course of six months to elicit immune responses against the conserved fusion peptide. Antibody specificities and GC responses were tracked longitudinally using electron microscopy polyclonal epitope mapping (EMPEM) and lymph node fine-needle aspirates, respectively. Application of cryoEMPEM delineated key residues for on-target and off-target responses that can drive the next round of structure-based vaccine design.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327030PMC
http://dx.doi.org/10.1101/2023.06.26.545779DOI Listing

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