AI Article Synopsis
- - The organ-on-chip model combines the advantages of in vitro and in vivo systems, offering a more accurate representation of biological processes.
- - The kidney-on-chip aims to mimic the structure and properties of kidney nephrons, using small, parallel channels made of collagen I for cell perfusion and structural support.
- - This microfluidic device is designed to enhance the study of kidney diseases, particularly focusing on conditions like polycystic kidney disease, by allowing researchers to investigate cellular interactions and mechanics in a controlled environment.
Article Abstract
The organ-on-chip model offers versatility and modularity of in vitro models while approaching the biological fidelity of in vivo models. We propose a method to build a perfusable kidney-on-chip aiming at reproducing key features of the densely packed segments of nephrons in vitro; such as their geometry, their extracellular matrix, and their mechanical properties. The core of the chip is made of parallel tubular channels molded into collagen I that are as small as 80 μm in diameter and as close as 100 μm apart. These channels can further be coated with basement membrane components and seeded by perfusion of a suspension of cells originating from a given segment of the nephron. We optimized the design of our microfluidic device to achieve high reproducibility regarding the seeding density of the channels and high fluidic control of the channels. This chip was designed as a versatile tool to study nephropathies in general, contributing to building ever better in vitro models. It could be particularly interesting for pathologies such as polycystic kidney diseases where mechanotransduction of the cells and their interaction with adjacent extracellular matrix and nephrons may play a key role.
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| http://dx.doi.org/10.1007/978-1-0716-3179-9_7 | DOI Listing |
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