gen. nov., sp. nov and sp. nov., isolated from human gut microbiota.

Two novel bacterial isolates were cultured from faecal samples of patients attending the Breast Care clinic at the Norwich and Norfolk University Hospital. Strain LH1062 was isolated from a 58-year-old female diagnosed with invasive adenocarcinoma with ductal carcinoma . Strain LH1063 was isolated from a healthy 51-year-old female. Isolate LH1062 was predicted to be a potential novel genus most closely related to , whilst LH1063 was predicted to be a novel species belonging to . Both strains were characterized by polyphasic approaches including 16S rRNA gene analysis, core-genome analysis, average nucleotide identity (ANI) comparisons and phenotypic analysis. Initial screening of the 16S rRNA gene of LH1062 returned a nucleotide identity of 93.4 % to . For LH1063, nucleotide identity was a 92.6 % to . Further investigations showed that LH1062 had a genome size of 2.9 Mb and G+C content of 31.3 mol %. LH1063 had a genome size of 3.3Mb and G+C content of 39.2 mol %. Digital DNA-DNA hybridization (dDDH) and ANI values of LH1062 with its closest relative, JCM 10604, were 20.9 and 79.54 %, respectively. For LH1063, the dDDH and ANI values with its closest relative, 177, were 19.3 and 77.81 %, respectively. Phenotypic testing confirmed that LH1062 could not be matched to a known validly published isolate in any database; thereby indicating a novel genus for which the name gen. nov. is now proposed with LH1062 (=DSM 114537=NCTC 14686) being the type strain of the proposed novel species sp. nov. Strain LH1063 (=DSM 114538=NCTC 14698) fits within the genus and, it being the third species within this genus, the name sp. nov. is proposed.