Temperature-regulated metabolites of inhibited reproduction of pinewood nematode .

The pinewood nematode is an invasive and destructive pathogen in forestry. AHPC29 was previously found to have nematicidal activity on . The effect of AHPC29 growth temperature on inhibition is unknown. Here we show that AHPC29 cultured at 15°C or 25°C, but not 37°C, inhibited reproduction. Metabolomic analysis found 31 up-regulated metabolites as potential effective substances in this temperature-related difference, with five of them were tested to be effective in inhibiting reproduction. Among the five metabolites, salsolinol was further verified in bacterial cultures with effective inhibition concentrations. This study found the inhibition of AHPC29 on reproduction was temperature regulated and the differently expressed metabolites salsolinol played roles in this temperature-regulated effect, which implies the capability of and its metabolites as promising new agents for the management of .