AI Article Synopsis
- Water-assisted laser desorption/ionization mass spectrometry (WALDI-MS), also known as SpiderMass, is a new method for analyzing biological samples in real-time using an infrared laser to excite water molecules.
- The technique utilizes water as a natural matrix to help release and ionize various biomolecules, including metabolites and lipids, from tissue samples.
- Recently, WALDI-MS has been developed into an imaging tool capable of producing both 2D and 3D images for ex vivo and in vivo studies, with specific guidelines for optimizing the imaging process.
Article Abstract
Water-assisted laser desorption/ionization mass spectrometry (WALDI-MS), also known as SpiderMass, is an emerging ambient ionization technique for in vivo and real-time analysis. It employs a remote infrared (IR) laser tuned to excite the most intense vibrational band (O-H) of water. The water molecules act as an endogenous matrix leading to the desorption/ionization of a variety of biomolecules from tissues, particularly metabolites and lipids. WALDI-MS was recently advanced into an imaging modality for ex vivo 2D sections and 3D in vivo real-time imaging. Here, we describe the methodological aspects for performing 2D and 3D imaging experiments with WALDI-MSI and the parameters for optimizing the image acquisition.
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| http://dx.doi.org/10.1007/978-1-0716-3319-9_8 | DOI Listing |
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