A PHP Error was encountered

Severity: Warning

Message: file_get_contents(https://...@gmail.com&api_key=61f08fa0b96a73de8c900d749fcb997acc09&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests

Filename: helpers/my_audit_helper.php

Line Number: 176

Backtrace:

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url

File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML

File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global

File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword

File: /var/www/html/index.php
Line: 316
Function: require_once

Purification, Expression, and Characterization of Sarcoplasmic Calcium Binding Protein: A Novel Allergen of . | LitMetric

Purification, Expression, and Characterization of Sarcoplasmic Calcium Binding Protein: A Novel Allergen of .

J Agric Food Chem

College of Food Science and Engineering, Ocean University of China, Huangdao Campus, Qingdao City, Shandong Province 266400, PR China.

Published: July 2023

Swimming crab (), a crucial valuable crustacean, is a common factor causing food allergy. However, studies on allergens of are scarce. In this study, the sarcoplasmic calcium binding protein (SCP) of was expressed in , purified with affinity chromatography, and the IgE-binding activity was evaluated through serological analyses. Further, the structure, physicochemical properties, and cross-reactivity were assessed via bioinformatics, immunologic, and spectroscopy techniques. The results indicated that SCP was an allergen displaying strong IgE-binding capacity, composed of 60% α-helix. It presented good immunologic and structural stability at 4-70 °C and pH 3-10, and only exhibited high IgG cross-reactivity among crustaceans, without cross-reactivity with other species tested. These results establish the foundations for further studies on SCP and are promising to promote the development of specific crustacean allergen detection and precise allergy diagnosis.

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.jafc.3c02043DOI Listing

Publication Analysis

Top Keywords

sarcoplasmic calcium
8
calcium binding
8
binding protein
8
purification expression
4
expression characterization
4
characterization sarcoplasmic
4
protein novel
4
novel allergen
4
allergen swimming
4
swimming crab
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!