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A combinatorial droplet microfluidic device integrated with mass spectrometry for enzyme screening. | LitMetric

AI Article Synopsis

  • Mass spectrometry (MS) is a powerful tool for detecting chemical species but has limitations in processing speed, which can be improved by integrating it with microfluidics.
  • The Drop-NIMS platform combines a microfluidic device with a unique MS technique that allows for the random mixing of droplets to create a library of enzymatic reactions directly on the NIMS surface, minimizing handling.
  • This method was successfully used to quickly screen low-volume enzymatic reactions, identifying various glycoside hydrolases relevant to industries like food and biofuels, demonstrating its versatility and ease of use for analyzing small molecule metabolites.

Article Abstract

Mass spectrometry (MS) enables detection of different chemical species with a very high specificity; however, it can be limited by its throughput. Integrating MS with microfluidics has a tremendous potential to improve throughput and accelerate biochemical research. In this work, we introduce Drop-NIMS, a combination of a passive droplet loading microfluidic device and a matrix-free MS laser desorption ionization technique called nanostructure-initiator mass spectrometry (NIMS). This platform combines different droplets at random to generate a combinatorial library of enzymatic reactions that are deposited directly on the NIMS surface without requiring additional sample handling. The enzyme reaction products are then detected with MS. Drop-NIMS was used to rapidly screen enzymatic reactions containing low (on the order of nL) volumes of glycoside reactants and glycoside hydrolase enzymes per reaction. MS "barcodes" (small compounds with unique masses) were added to the droplets to identify different combinations of substrates and enzymes created by the device. We assigned xylanase activities to several putative glycoside hydrolases, making them relevant to food and biofuel industrial applications. Overall, Drop-NIMS is simple to fabricate, assemble, and operate and it has potential to be used with many other small molecule metabolites.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484474PMC
http://dx.doi.org/10.1039/d2lc00980cDOI Listing

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