AI Article Synopsis
- The study focuses on how the locus coeruleus (LC) and noradrenergic transmission relate to sudden unexpected death in epilepsy (SUDEP).
- It outlines a protocol to modulate the noradrenergic pathway from the LC to the heart to potentially prevent SUDEP in specific mouse models.
- Key steps include creating SUDEP models, recording calcium signals, monitoring ECGs, and analyzing neurotransmitter components and neuron destruction.
Article Abstract
The locus coeruleus (LC) and noradrenergic neurotransmission are involved in the regulation of sudden unexpected death in epilepsy (SUDEP). Here, we present a protocol for modulating the noradrenergic pathway from LC to heart to prevent SUDEP in acoustic and pentylenetetrazole-induced DBA/1 mouse models of SUDEP. We describe steps for constructing SUDEP models, calcium signal recording, and electrocardiogram monitoring. We then detail measurement of tyrosine hydroxylase content and activity, β1 and p-β1-AR content, and destruction of LC neurons. For complete details on the use and execution of this protocol, please refer to Lian et al..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10336304 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102403 | DOI Listing |
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