Deciphering the enigma of RNA editing in the ATP1_alpha subunit of ATP synthase in .

There is evidence that RNA editing is related to plant cellular stress as well as electron transport organelles, such as mitochondria. The mitochondrial gene encodes the alpha-subunit of Atp synthase. Control as well as two periods of drought stress treatments were analyzed in the cDNAs generated from the mitochondrial gene of two cultivars of [Giza 168 (G168) and Gemmiza 10 (GM10)]. Following RNA-seq data assembly, cDNAs from the control (acc. no. OQ129415), 2-hour (acc. no. OQ129416), and 12-hour (acc. no. OQ129417) time points of the cultivar G168 were obtained. Control (acc. no. OQ129419), 2-hour (acc. no. OQ129420), and 12-hour (acc. no. OQ129421) samples all included reconstructed transcripts from Gemmiza 10. transcripts were assembled using the wheat gene (acc. no. NC_036024). RNA-seq raw data was utilized to identify 11 RNA editing sites in in the tolerant cultivar Giza168 and 6 in the sensitive cultivar Gemmiza10. The significant difference in RNA editing observed between control and drought stress conditions in sites led to synonymous amino acids. This led to no change in tertiary structure between tolerant and sensitive cultivars. But the change was focused between produced protein and its correspondence sequence on DNA.