AI Article Synopsis
- Current methods for detecting protein modifications, like phosphates, struggle with measuring single molecules and distinguishing closely spaced sites.
- The study introduces a technique that uses nanopores to analyze individual immunopeptide sequences that have cancer-related phosphate variants.
- This method successfully differentiates between peptides with one or two closely spaced phosphate groups with 95% accuracy for each single molecule read.
Article Abstract
Current methods to detect post-translational modifications of proteins, such as phosphate groups, cannot measure single molecules or differentiate between closely spaced phosphorylation sites. We detect post-translational modifications at the single-molecule level on immunopeptide sequences with cancer-associated phosphate variants by controllably drawing the peptide through the sensing region of a nanopore. We discriminate peptide sequences with one or two closely spaced phosphates with 95% accuracy for individual reads of single molecules.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11189593 | PMC |
| http://dx.doi.org/10.1038/s41587-023-01839-z | DOI Listing |
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