Small noncoding RNAs are an important class of regulatory RNAs in bacteria, often regulating responses to changes in environmental conditions. OxyS is a 110 nt, stable, -encoded small RNA found in and is induced by an increased concentration of hydrogen peroxide. OxyS has an important regulatory role in cell stress response, affecting the expression of multiple genes. In this work, we investigated the structure of OxyS and the interaction with mRNA using nuclear magnetic resonance spectroscopy, small-angle X-ray scattering, and unbiased molecular dynamics simulations. We determined the secondary structures of isolated stem-loops and confirmed their structural integrity in OxyS. Unexpectedly, stem-loop SL4 was identified in the region that was predicted to be unstructured. Three-dimensional models of OxyS demonstrate that OxyS adopts an extended structure with four solvent-exposed stem-loops, which are available for interaction with other RNAs and proteins. Furthermore, we provide evidence of base-pairing between OxyS and mRNA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578473 | PMC |
| http://dx.doi.org/10.1261/rna.079618.123 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Invisible but Insidious Effects of Microplastics.
Molecules
December 2024
N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry, Siberian Branch of Russian Academy of Sciences, Lavrentjev Avenue 9, 630090 Novosibirsk, Russia.
Increasing evidence on the adverse health impacts of microplastics (MPs) is available, but their associated risks to the well-being of humans and long-term impacts are poorly understood. An indicator of the remote effects of MPs may be their influence on the rate of aging. To assess the effects of MPs on the aging process, we used accelerated senescence OXYS rats that develop a complex of geriatric diseases.
View Article and Find Full Text PDFSmall RNA OxyS induces resistance to aminoglycosides during oxidative stress by controlling Fe-S cluster biogenesis in .
Proc Natl Acad Sci U S A
November 2024
CNRS, Aix-Marseille Université, Laboratoire de Chimie Bactérienne, UMR7283, Institut de Microbiologie de la Méditérannée, Institut Microbiologie, Bioénergies et Biotechnologie, Marseille F-13009, France.
Fe-S clusters are essential cofactors involved in many reactions across all domains of life. Their biogenesis in and other enterobacteria involves two machineries: Isc and Suf. Under conditions where cells operate with the Suf system, such as during oxidative stress or iron limitation, the entry of aminoglycosides is reduced, leading to resistance to these antibiotics.
View Article and Find Full Text PDFThe Expression of Genes , , and in Distinct Regions of the Heart and Its Possible Contribution to the Development of Hypertension.
Biomedicines
October 2024
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Akad. Lavrentiev Ave., Novosibirsk 630090, Russia.
Background: It is believed that alterations in the functioning of the cytochrome P450 (CYP), which participates in metabolic transformations of endogenous polyunsaturated fatty acids (PUFAs) (with the formation of cardioprotective or cardiotoxic products), affects the development of age-related cardiovascular diseases and reduces the effectiveness of some cardioselective drugs. For example, CYP2J2 activation or CYP1B1 inhibition protects against the cardiovascular toxicity of anticancer drugs. It is currently unclear whether CYPs capable of metabolizing arachidonic acid and ω-3 PUFAs to vasodilatory and vasoconstrictive derivatives are expressed in all heart regions.
View Article and Find Full Text PDFThe effect of β-ionone on bacterial cells: the use of specific lux-biosensors.
Res Microbiol
September 2024
Complex of NBICS Technologies, National Research Center "Kurchatov Institute", Kurchatov sq. 2, 123182 Moscow, Russia; Kurchatov Center for Genome Research, National Research Center "Kurchatov Institute", Kurchatov sq. 2, 123182 Moscow, Russia. Electronic address:
Article Synopsis
- The study highlights the diverse biological effects of β-ionone, a volatile organic compound, on bacterial cells, specifically focusing on Escherichia coli and Bacillus subtilis.
- It was found that β-ionone induces oxidative stress in E. coli through the OxyR/OxyS regulatory system, but not through the SoxR/SoxS system, suggesting a specific response to the compound.
- Additionally, at high concentrations, β-ionone can cause protein and DNA damage, while showing no oxidative stress effects in Bacillus subtilis, indicating the varying impact of β-ionone across different bacterial species.
Retinoprotective Effect of SkQ1, Visomitin Eye Drops, Is Associated with Suppression of P38 MAPK and ERK1/2 Signaling Pathways Activity.
Biochemistry (Mosc)
February 2024
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, 630090, Russia.
Visomitin eye drops are the first and, so far, the only drug based on SkQ1 - the mitochondria-targeted antioxidant 10-(6'-plastoquinonyl) decyltriphenylphosphonium, developed in the laboratories of Moscow State University under the leadership of Academician V. P. Skulachev.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!