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Detection of Zika Virus in and Mosquitoes Collected in Urban Forest Fragments in the Brazilian Amazon. | LitMetric

AI Article Synopsis

  • * The study focused on identifying ZIKV infection in mosquitoes from urban forests in Manaus, collecting a total of 905 samples from different mosquito species over several years.
  • * Findings revealed that 15% of certain mosquito pools tested positive for ZIKV, marking the first evidence of naturally infected mosquitoes in the Amazon area.

Article Abstract

Zika virus (ZIKV) is an RNA flavivirus ( family) endemic in tropical and subtropical regions that is transmitted to humans by () species mosquitoes. The two main urban vectors of ZIKV are and which can be found throughout Brazil. This study investigated ZIKV infection in mosquito species sampled from urban forest fragments in Manaus (Brazilian Amazon). A total of 905 non-engorged female (22 specimens) and (883 specimens) were collected using BG-Sentinel traps, entomological hand nets, and Prokopack aspirators during the rainy and dry seasons between 2018 and 2021. All pools were macerated and used to inoculate C6/36 culture cells. Overall, 3/20 (15%) and 5/241 (2%) pools screened using RT-qPCR were positive for ZIKV. No supernatants from were positive for ZIKV (0%), and 15 out of 241 (6.2%) pools were positive. In this study, we provide the first-ever evidence of naturally infected with ZIKV in the Amazon region.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304349PMC
http://dx.doi.org/10.3390/v15061356DOI Listing

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