AI Article Synopsis
- Traditional methods for identifying infectious pathogens are complicated and slow, creating a significant health burden.
- This research developed multifunctional copolymers with rhodamine B dye using a method called atom transfer radical polymerization (ATRP), which allowed for efficient synthesis.
- The resulting biotinylated dye copolymers, when combined with antibodies or binding domains, showed enhanced fluorescence and specificity for bioimaging, suggesting their potential use as biosensors for detecting DNA, proteins, or bacteria.
Article Abstract
Infectious diseases caused by pathogens are a health burden, but traditional pathogen identification methods are complex and time-consuming. In this work, we have developed well-defined, multifunctional copolymers with rhodamine B dye synthesized by atom transfer radical polymerization (ATRP) using fully oxygen-tolerant photoredox/copper dual catalysis. ATRP enabled the efficient synthesis of copolymers with multiple fluorescent dyes from a biotin-functionalized initiator. Biotinylated dye copolymers were conjugated to antibody (Ab) or cell-wall binding domain (CBD), resulting in a highly fluorescent polymeric dye-binder complex. We showed that the unique combination of multifunctional polymeric dyes and strain-specific Ab or CBD exhibited both enhanced fluorescence and target selectivity for bioimaging of by flow cytometry and confocal microscopy. The ATRP-derived polymeric dyes have the potential as biosensors for the detection of target DNA, protein, or bacteria, as well as bioimaging.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10304923 | PMC |
| http://dx.doi.org/10.3390/polym15122723 | DOI Listing |
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