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Genome Sequence and Evaluation of Safety and Probiotic Potential of LPJZ-658. | LitMetric

AI Article Synopsis

  • - This study evaluates the safety and probiotic properties of LPJZ-658, a novel strain found to have a genome size of 3.26 Mbp and a GC content of 44.83%, identifying 3,254 putative open reading frames (ORFs).
  • - Whole genome analysis revealed a significant gene cluster for secondary metabolites and a putative bile saline hydrolase (BSH), which contribute to LPJZ-658's verified safety and probiotic characteristics.
  • - LPJZ-658 is non-toxic, does not cause hemolysis, is susceptible to various antibiotics, and shows resistance to acid and bile, along with strong antimicrobial properties against harmful gut pathogens, indicating its potential as a

Article Abstract

This study aims to systematically evaluate the safety of a novel LPJZ-658 explored on whole-genome sequence analysis, safety, and probiotic properties assessment. Whole genome sequencing results demonstrated that LPJZ-658 consists of 3.26 Mbp with a GC content of 44.83%. A total of 3254 putative ORFs were identified. Of note, a putative bile saline hydrolase (BSH) (identity 70.4%) was found in its genome. In addition, the secondary metabolites were analyzed, and one secondary metabolite gene cluster was predicted to consist of 51 genes, which verified its safety and probiotic properties at the genome level. Additionally, LPJZ-658 exhibited non-toxic and non-hemolytic activity and was susceptible to various tested antibiotics, indicating that LPJZ-658 was safe for consumption. Moreover, the probiotic properties tests confirm that LPJZ-658 also exhibits tolerance to acid and bile salts, preferably hydrophobicity and auto-aggregation, and excellent antimicrobial activity against both Gram-positive and Gram-negative gastrointestinal pathogens. In conclusion, this study confirmed the safety and probiotic properties of LPJZ-658, suggesting it can be used as a potential probiotic candidate for human and animal applications.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10301710PMC
http://dx.doi.org/10.3390/microorganisms11061620DOI Listing

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