Role of the IL-33/ST2 Activation Pathway in the Development of the Hepatic Fibrosis Induced by Granulomas in Mice.

eggs retained in host tissues induce innate cytokine release, contributing to the induction of Type-2 immune responses and granuloma formation, important to restrain cytotoxic antigens, but leading to fibrosis. Interleukin(IL)-33 participates in experimental models of inflammation and chemically induced fibrosis, but its role in -induced fibrosis is still unknown. To explore the role of the IL-33/suppressor of the tumorigenicity 2 (ST2) pathway, serum and liver cytokine levels, liver histopathology, and collagen deposition were comparatively evaluated in -infected wild-type (WT) and IL-33-receptor knockout (ST2) BALB/c mice. Our data show similar egg counts and hydroxyproline in the livers of infected WT and ST2 mice; however, the extracellular matrix in ST2 granulomas was loose and disorganised. Pro-fibrotic cytokines, such as IL-13 and IL-17, and the tissue-repairing IL-22 were significantly lower in ST2 mice, especially in chronic schistosomiasis. ST2 mice also showed decreased α-smooth muscle actin (α-SMA) expression in granuloma cells, in addition to reduced and mRNA levels and reticular fibres. Therefore, IL-33/ST2 signalling is essential for tissue repairing and myofibroblast activation during infection. Its disruption results in inappropriate granuloma organisation, partly due to the reduced type III and VI collagen and reticular fibre formation.