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Recreating the Trabecular Outflow Tissue on Implantable, Micropatterned, Ultrathin, Porous Polycaprolactone Scaffolds. | LitMetric

AI Article Synopsis

  • Glaucoma causes irreversible blindness due to increased pressure in the eye, damaging the optic nerve, mainly linked to the malfunction of trabecular meshwork (HTM) cells.
  • A promising treatment involves replacing damaged HTM cells with tissue-engineered substitutes using a biodegradable material called polycaprolactone (PCL).
  • The study showed that specific micropatterned PCL scaffolds could effectively support the growth and function of HTM cells, suggesting their potential for creating healthy ocular outflow tissue.

Article Abstract

Glaucoma, where increased intraocular pressure (IOP) leads to damage to the optic nerve and loss of sight, is amongst the foremost causes of irreversible blindness worldwide. In primary open angle glaucoma, the increased IOP is a result of the malfunctioning human trabecular meshwork (HTM) cells' inability to properly regulate the outflow of aqueous humor from the eye. A potential future treatment for glaucoma is to replace damaged HTM cells with a tissue-engineered substitute, thus restoring proper fluid outflow. Polycaprolactone (PCL) is a versatile, biodegradable, and implantable material that is widely used for cell culture and tissue engineering. In this work, PCL scaffolds were lithographically fabricated using a sacrificial process to produce submicron-thick scaffolds with openings of specific sizes and shapes (e.g., grid, hexagonal pattern). The HTM cell growth on gelatin-coated PCL scaffolds was assessed by scanning electron microscopy, tetrazolium metabolic activity assay, and cytoskeletal organization of F-actin. Expression of HTM-specific markers and ECM deposition were assessed by immunocytochemistry and qPCR analysis. Gelatin-coated, micropatterned, ultrathin, porous PCL scaffolds with a grid pattern supported proper HTM cell growth, cytoskeleton organization, HTM-marker expression, and ECM deposition, demonstrating the feasibility of using these PCL scaffolds to tissue-engineer implantable, healthy ocular outflow tissue.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10294786PMC
http://dx.doi.org/10.3390/bioengineering10060679DOI Listing

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