Whole Genome Sequencing and Phenotypic Analysis of Antibiotic Resistance in Isolates.

There is scarce knowledge regarding the antimicrobial resistance profile of Therefore, the objective of this research was to assess antimicrobial resistance in recently obtained clinical isolates and to identify the presence of antimicrobial resistance genes. Isolates were obtained from patients with periodontal or peri-implant diseases and confirmed by sequencing their 16S rRNA gene. Confirmed isolates had their genome sequenced by whole genome sequencing and their phenotypical resistance to nine antibiotics (amoxicillin clavulanate, amoxicillin, azithromycin, clindamycin, ciprofloxacin, doxycycline, minocycline, metronidazole, and tetracycline) tested by E-test strips. Antimicrobial resistance genes were detected in six of the eight isolates analyzed, of which five carried (32) and one (B). Overall, susceptibility to the nine antibiotics tested was high except for azithromycin in the isolate that carried (B). Moreover, susceptibility to tetracycline, doxycycline, and minocycline was lower in those isolates that carried (32). The genetic surroundings of the detected genes suggested their inclusion in mobile genetic elements that might be transferrable to other bacteria. These findings suggest that, despite showing high susceptibility to several antibiotics, might obtain new antimicrobial resistance traits due to its acceptance of mobile genetic elements with antibiotic resistance genes in their genome.